Improved Production of Fructooligosaccharides (scFOS)
Short chain fructooligosaccharides (scFOS) are prebiotics used as a food supplement to improve dietary health in humans and animals. FOS are also used in the food industry as low-calorie sweeteners and added to food products to improve their organoleptic properties.
Global demand for FOS is growing and this invention modifies B- fructofuranosidase enzymes to enhance their performance in producing FOS under industrial conditions.
The invention modifies B-fructofuranosidases for improved enzyme activity, relative to the parent enzyme.
An existing beta-fructofuranosidase (fopA) was modified. Comparison of the modified enzyme with the "wild-type" showed a 26% reduction in the time required to convert a given amount of sucrose into scFOS of a composition resembling that of a commercial scFOS product. Experimental data also show that the enzyme has improved thermostability. Thus a given amount of enzyme can produce scFOS in a shorter time or, for a given batch time, less enzyme is required to achieve the same results.
- Producers of scFOS and enzymes.
- Companies employing scFOS as nutraceutical supplements in pharmaceutical formulations, dairy products, animal feeds.
- The health food industry.
- Reduction in run times for batch production of scFOS.
- Reduction in enzyme and scFOS production costs.
- Improved thermolability of the existing fopA enzyme.
- Improved commercial viability of the fopA enzyme.